AnaSpec Introduces Industry's First Fluorimetric Assay - SensoLyte(R) Cathepsin G Kits

Top Quote AnaSpec, Eurogentec Group, is pleased to announce the release of the industry's first fluorimetric kits for Cathepsin G, the SensoLyte® 520 Cathepsin G Assay Kit and SensoLyte® Rh110 Cathepsin G Assay Kit. These long-wavelength, homogeneous assays are ideal for the screening of enzyme inhibitors and activators or for continuous assay of Cathepsin G activity. End Quote
  • (1888PressRelease) February 15, 2011 - The SensoLyte® 520 Cathepsin G Assay Kit contains a FRET-based (HiLyte Fluor™ 488/QXL™ 520) substrate and the SensoLyte® Rh110 Cathepsin G Assay Kit, a fluorogenic substrate labeled with Rh110. These 2 substrates have excitation/emission at the green wavelengths of 490/520 nm. The longer wavelength spectra and higher extinction coefficient of HiLyte Fluor™ 488 and Rh110 provide greater sensitivity and less interference from other reaction components.

    Cathepsin G is a neutrophil serine protease, stored in cytoplasmic granules and secreted into the extra- and pericellular space upon neutrophil activation.1 Cathepsin G plays important role in bacterial defense. It acts in combination with reactive oxygen species to help degrade engulfed microorganisms inside phagolysosomes.2,3 Besides bactericidal effect, Cathepsin G regulates the inflammatory response by specifically altering the function of cytokines and chemokines.4 Because of its role in host defense, Cathepsin G is of interest as a potential therapeutic target.5

    1. Egesten, A. et al. Blood 83, 2985 (1994).
    2. Korkmaz, B. et al. Biochimie. 90, 227 (2008).
    3. Sieńczyk M. et al. Bioorg Med Chem. 19,8863 (2008).
    4. Pham, CT. Nat Rev Immunol. 6, 541 (2006).
    5. Korkmaz, B. et al. Pharmacol Rev. 62, 726 (2010).
    6. Lesner, A. et al. Anal Biochem. 375, 306 (2008).
    7. Greco, MN. et al. J Am Chem Soc. 124, 3810 (2002).

    About AnaSpec, Eurogentec Group

    AnaSpec, Eurogentec Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. As a subsidiary of Eurogentec, AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, oligonucleotides, and qPCRs. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.

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