Thermo Fisher Scientific Announces a High-Resolution Separation of Polysialic Acid

Top Quote Thermo Fisher Scientific introduces a high-performance anion-exchange with pulsed amperometric detection method for the analysis of polysialic acids. End Quote
  • San Jose, CA (1888PressRelease) October 25, 2012 - Thermo Fisher Scientific is pleased to announce a high-performance anion-exchange with pulsed amperometric detection (HPAE-PAD) method for the analysis of a homologous series of polysialic acids. Application Note 1013: Polysialic Acid Analysis: Separating Polymers with High Degrees of Polymerization demonstrates that this new approach achieves better resolution than existing methods. Using a specially developed column, this method resolves homologues of colominic acid, a polysialic acid, with a degree of polymerization (DP) ≤100 in 70 minutes (with a sodium acetate gradient) and ≤140 in 90 minutes (with a sodium nitrate gradient).

    This new method surpasses the maximum DP of ~60 for acetate and ~80 for nitrate that can be achieved with other anion-exchange columns. The Thermo Scientific Dionex CarboPac PA200 column resolves high DP polymers better than other columns typically used for this application. The small particle size of the substrate (5.5 μm) and microbead (43 nm) enable the Dionex CarboPac™ PA200 column to perform these higher-resolution separations.

    This application note and many others can be found at under the Documents tab.

    About Thermo Fisher Scientific
    Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity. Visit

    All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

    Social Media Contact Information
    Sonya Pelia
    1214 Oakmead Parkway
    Sunnyvale, CA 94085 USA
    Phone: +1 408 481 4172
    sonya.pelia ( @ ) thermofisher dot com

  • FB Icon Twitter Icon In-Icon
Contact Information