(1888PressRelease)
February 23, 2008 - Trevigen launches High-Throughput PARP/Apoptosis Assays ideal for measuring the activity of PARP in 96-well plates. Cell extracts can be obtained using our newly-developed, higher-throughput procedure before, during and after apoptosis induction.
During apoptosis, PARP-1 which catalyzes the NAD dependent addition of poly (ADP-ribose) (PAR) onto various cytoplasmic and nuclear proteins, is cleaved from about 116 kDa to 85 kDa. Trevigen's HT PARP/Apoptosis Assay is ideal for measuring the activity of PARP in cell extracts before and during apoptosis. The HT PARP/Apoptosis Assay is an ELISA which semi-quantitatively detects PAR deposited onto immobilized histone proteins in a 96-well format. An anti-PAR monoclonal antibody, goat anti-mouse IgG-HRP conjugate, and HRP substrate are used to generate a colorimetric signal. Thus, absorbance correlates with PARP activity. Etoposide is a topoisomerase II inhibitor that stabilizes this enzyme after it cleaves DNA. It is included as a control apoptosis inducer.
Trevigen offers two formats of the HT PARP/Apoptosis Assay: Cat#4684-096-K (Colorimetric) and Cat# 4685-096-K (Chemiluminescent). Both assays feature non-radioactive formats and high-throughput 96 test size, as well as sensitivity down to 0.1 mUnits of PARP--equivalent to less than 500 cells/well. Additional
histone-coated strip wells (Cat# 4677-096-P) are available separately.
For more details about this product, contact Trevigen by phone at 800-873-8443, email info ( @ ) trevigen dot com or visit the Trevien website www.trevigen.com.
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